Difference between revisions of "Part:BBa K1909009"

 
 
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<partinfo>BBa_K1909009 short</partinfo>
 
<partinfo>BBa_K1909009 short</partinfo>
  
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BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6]  ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).
  
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===Usage and Biology===
 
===Usage and Biology===
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The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.
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===Functional Parameters===
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This combination has the lowest expression of the three combinations BBa_K1909007, BBa_K1909008 and BBa_K1909009.
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Testing the combination showed that the cells grew very, very slow (for miniprep culture more than 48 hours, for colonies on LB-agar plates more than 72 hours).
  
 
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Latest revision as of 22:45, 19 October 2016


Weak Anderson / mTaz

BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).

Usage and Biology

The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.

Functional Parameters

This combination has the lowest expression of the three combinations BBa_K1909007, BBa_K1909008 and BBa_K1909009. Testing the combination showed that the cells grew very, very slow (for miniprep culture more than 48 hours, for colonies on LB-agar plates more than 72 hours).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174