Difference between revisions of "Part:BBa K1915004:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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TBD
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To design the CBDA synthase with the LacI + pL promoter, the CBDA synthase was moved through pSB1A3 before re-entering pSB1C3. The RFP was first digested out of the pSB1A3 and using the IDT constitutive CBDA synthase coding sequence, the sequence was ligated into pSB1A3 with the Promotors. After ligation and confirmation of the part, we digested the CBDA synthase and the promoter out of pSB1A3 and ligated back into pSB1C3 vector. Withe the strong promoter, expressing CBDA synthase should be inducible by IPTG from E.Coli DH5-alpha-Z1 over a >600-fold range. Furthermore, with the LacI included in the promoter, the coding of CBDA synthase can also be repress by lac inhibition.
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===Source===
 
===Source===

Latest revision as of 16:26, 29 October 2016


CBDAs with Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 622
    Illegal NgoMIV site found at 1201
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design the CBDA synthase with the LacI + pL promoter, the CBDA synthase was moved through pSB1A3 before re-entering pSB1C3. The RFP was first digested out of the pSB1A3 and using the IDT constitutive CBDA synthase coding sequence, the sequence was ligated into pSB1A3 with the Promotors. After ligation and confirmation of the part, we digested the CBDA synthase and the promoter out of pSB1A3 and ligated back into pSB1C3 vector. Withe the strong promoter, expressing CBDA synthase should be inducible by IPTG from E.Coli DH5-alpha-Z1 over a >600-fold range. Furthermore, with the LacI included in the promoter, the coding of CBDA synthase can also be repress by lac inhibition.

Source

TBD

References