Difference between revisions of "Part:BBa K1909008"

 
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<partinfo>BBa_K1909008 short</partinfo>
 
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BBa_K1909008 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).
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===Usage and Biology===
 
===Usage and Biology===
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The receptor mTaz is based on the chemotaxis receptor Taz, part BBa_C0082, capable of sensing aspartate and in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608006 and BBa_C0082 is part BBa_K1909011.
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This combination has a higher expression as BBa_K1909009 and a lower expression as BBa_K1909007.
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===Functional Parameters===
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Testing the combination showed that cells grew slowly (for miniprep culture more than 24 hours).
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We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor.
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Latest revision as of 22:48, 19 October 2016


Medium Anderson / mTaz

BBa_K1909008 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).

Usage and Biology

The receptor mTaz is based on the chemotaxis receptor Taz, part BBa_C0082, capable of sensing aspartate and in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608006 and BBa_C0082 is part BBa_K1909011. This combination has a higher expression as BBa_K1909009 and a lower expression as BBa_K1909007.

Functional Parameters

Testing the combination showed that cells grew slowly (for miniprep culture more than 24 hours). We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 173