Difference between revisions of "Part:BBa K2172000"

 
 
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<partinfo>BBa_K2172000 short</partinfo>
 
<partinfo>BBa_K2172000 short</partinfo>
  
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This part contains a GST label, a thrombin restriction enzyme cleavage site and a GFP detector. The GST label can be used to purify proteins. The thrombin site can be used to digest the part into two. The GFP detector can verify where the part is being expressed or not. This part can be used to synthesize other parts that require to be purified and be checked for its expression.
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This part contains a GST label, a thrombin protease cleavage site and a GFP detector. The GST label can be used to purify proteins. The thrombin site can be used to digest the part into two. The GFP detector can verify where the part is being expressed or not. This part can be used to synthesize other parts that require to be purified and be checked for its expression.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 15:14, 19 October 2016


GST-Thrombin-GFP

This part contains a GST label, a thrombin protease cleavage site and a GFP detector. The GST label can be used to purify proteins. The thrombin site can be used to digest the part into two. The GFP detector can verify where the part is being expressed or not. This part can be used to synthesize other parts that require to be purified and be checked for its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85