Difference between revisions of "Part:BBa K1923005"
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<partinfo>BBa_K1923005 short</partinfo> | <partinfo>BBa_K1923005 short</partinfo> | ||
− | + | This basic part encodes an enzyme called thymidine kinase can function as a selectable and counterselectable marker gene in yeast. In certain medium condition TK positive yeast will survive while in another medium condition only TK negative yeast can grow <sup>[1]</sup>. | |
+ | |||
+ | For more information, please refer to the Wiki page of Team Tsinghua. | ||
+ | |||
+ | <strong>Reference:</strong> | ||
+ | <br> | ||
+ | [1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866. | ||
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Latest revision as of 20:15, 18 October 2016
Thymidine kinase encoding gene
This basic part encodes an enzyme called thymidine kinase can function as a selectable and counterselectable marker gene in yeast. In certain medium condition TK positive yeast will survive while in another medium condition only TK negative yeast can grow [1].
For more information, please refer to the Wiki page of Team Tsinghua.
Reference:
[1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]