Difference between revisions of "Part:BBa K1981005:Design"

 
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===Design Notes===
 
===Design Notes===
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Point mutation was performed to mutate the restriction site of XbaI at 192 bp. Condon optimization was performed to meet the
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Point mutation was performed to mutate the restriction site of EcoRI at 1037 bp.
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manufacture requirement of IDT to synthesize the DNA fragment. 
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−
 
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−
 
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===Source===
 
===Source===
  
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lsrFG source
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Sequence was acquired from genomic of <i>Escherichia coli</i> strain K12. Part was chemically synthesized.
  
 
===References===
 
===References===

Latest revision as of 17:22, 14 October 2016


lsrFG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutation was performed to mutate the restriction site of EcoRI at 1037 bp.

Source

Sequence was acquired from genomic of Escherichia coli strain K12. Part was chemically synthesized.

References