Difference between revisions of "Part:BBa K1893014:Design"
(→Source) |
|||
| (2 intermediate revisions by one other user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This sequence was obtained through the use of inverse PCR on the 'j23119-AntiSTAR/pSB1C3' construct (BBa_K1893011) to remove the constitutive promoter j23119. | |
| + | Anti-STAR forward primer: AGTTTTTACAGTGAATTGTTTTAATTAGTTG | ||
| + | Prefix Reverse primer: CTCTAGAAGCGGCCGCG | ||
===Source=== | ===Source=== | ||
| − | + | Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time using the [http://rna.urmc.rochester.edu/RNAstructure.html RNAstructure] software. | |
===References=== | ===References=== | ||
| + | |||
| + | Imperial iGEM 2016 | ||
Latest revision as of 15:35, 27 October 2016
anti-STAR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was obtained through the use of inverse PCR on the 'j23119-AntiSTAR/pSB1C3' construct (BBa_K1893011) to remove the constitutive promoter j23119.
Anti-STAR forward primer: AGTTTTTACAGTGAATTGTTTTAATTAGTTG
Prefix Reverse primer: CTCTAGAAGCGGCCGCG
Source
Anti-STAR is a sequence that Imperial iGEM 2016 have designed for the first time using the [http://rna.urmc.rochester.edu/RNAstructure.html RNAstructure] software.
References
Imperial iGEM 2016