Difference between revisions of "Part:BBa K1893012"
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| − | + | To regulate STAR activity, we designed an inhibitory RNA sequence (Anti-STAR). The Anti-STAR sequence is identical to a portion of the pAD1.S5 attenuator sequence, and is therefore also complementary to STAR. Anti-STAR has a higher affinity for STAR than the pAD1 attenuator does, so, when transcribed, Anti-STAR sequesters STAR and prevents it from interacting with the target sequence and activating transcription. | |
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Latest revision as of 16:02, 27 October 2016
Constitutive STAR and anti-STAR (J23119+STAR+anti-STAR)
To regulate STAR activity, we designed an inhibitory RNA sequence (Anti-STAR). The Anti-STAR sequence is identical to a portion of the pAD1.S5 attenuator sequence, and is therefore also complementary to STAR. Anti-STAR has a higher affinity for STAR than the pAD1 attenuator does, so, when transcribed, Anti-STAR sequesters STAR and prevents it from interacting with the target sequence and activating transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 154
Illegal NheI site found at 177 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]