Difference between revisions of "Part:BBa K1918109:Design"

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===Design Notes===
 
===Design Notes===
−
ligation from [pwx227(EcoRI&XbaI)][pwx268(WL16049&WL16052)]
+
AIM: the expression of the total fluorescent protein EBFP2 could be induced by Dox;
 +
ELEMENTS: EBFP2, Dox-induced promoter TRE
  
 
===Source===
 
===Source===
−
 
+
PCR from the existing plasmid in xie lab
−
 
+
−
 
+
  
 
===References===
 
===References===

Latest revision as of 11:41, 14 October 2016


TRE-Kozak-EBFP2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AIM: the expression of the total fluorescent protein EBFP2 could be induced by Dox; ELEMENTS: EBFP2, Dox-induced promoter TRE

Source

PCR from the existing plasmid in xie lab

References