Difference between revisions of "Part:BBa K1918105:Design"

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===Design Notes===
 
===Design Notes===
−
ligation from [pwx240(KpnI&XbaI)][pwx236(KpnI&XbaI)]
+
AIM: EBFP2C(155-238) could be expressed in cell all time; ELEMENTS: EBFP2C(155-238), CMV
  
 
===Source===
 
===Source===
−
[pwx240(KpnI&XbaI)]
+
PCR from the existing plasmid in xie lab
−
[pwx236(KpnI&XbaI)]
+
  
 
===References===
 
===References===

Latest revision as of 12:32, 14 October 2016


CMV-Kozak-EBFP2C(155-238)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

AIM: EBFP2C(155-238) could be expressed in cell all time; ELEMENTS: EBFP2C(155-238), CMV

Source

PCR from the existing plasmid in xie lab

References