Difference between revisions of "Part:BBa K1918109"

 
 
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<partinfo>BBa_K1918109 parameters</partinfo>
 
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<p>In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.</p>
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<p>Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.</p>
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Latest revision as of 22:05, 19 October 2016


TRE-Kozak-EBFP2

This part is consist of the total coding region of the fluorescent protein EBFP2 and the TRE promoter. Thus, the expression of EBFP2 would be induced by Dox.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 1111
  • 1000
    COMPATIBLE WITH RFC[1000]


Template:Tsinghua-A

    In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.

    Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.