Difference between revisions of "Part:BBa K1918108:Design"

 
(→‎Source)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
 
+
AIM: EBFP2N(1-154) could be expressed in cell all time;
−
 
+
ELEMENTS: EBFP2N(1-154), CMV
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
PCR from the existing plasmid in xie lab
−
 
+
−
 
+
  
 
===References===
 
===References===

Latest revision as of 12:41, 14 October 2016


CMV-Kozak-EBFP2N(1-154)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 756
    Illegal SpeI site found at 84
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 756
    Illegal BamHI site found at 739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 756
    Illegal XbaI site found at 1236
    Illegal SpeI site found at 84
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 717


Design Notes

AIM: EBFP2N(1-154) could be expressed in cell all time; ELEMENTS: EBFP2N(1-154), CMV

Source

PCR from the existing plasmid in xie lab

References