Difference between revisions of "Part:BBa K1918108"
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<partinfo>BBa_K1918108 short</partinfo> | <partinfo>BBa_K1918108 short</partinfo> | ||
− | This part is consist of | + | This part is consist of EBFP2N(1-154) and the CMV promoter. EBFP2N(1-154) is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. Driven by CMV promoter, the EBFP2N(1-154) could be expressed all time. |
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<partinfo>BBa_K1918108 parameters</partinfo> | <partinfo>BBa_K1918108 parameters</partinfo> | ||
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+ | {{Tsinghua-A}} | ||
+ | <html> | ||
+ | <div class="column full_size"> | ||
+ | <ul> | ||
+ | |||
+ | <p>In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.</p> | ||
+ | |||
+ | <div class="column full_size" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/f/fa/T--Tsinghua-A--pro0010.jpg"> | ||
+ | </div> | ||
+ | <div class="column full_size" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/4/44/T--Tsinghua-A--results0002.jpg"> | ||
+ | </div> | ||
+ | |||
+ | <p>Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.</p> | ||
+ | </ul> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </html> |
Latest revision as of 22:05, 19 October 2016
CMV-Kozak-EBFP2N(1-154)
This part is consist of EBFP2N(1-154) and the CMV promoter. EBFP2N(1-154) is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2. Driven by CMV promoter, the EBFP2N(1-154) could be expressed all time.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 756
Illegal SpeI site found at 84 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 756
Illegal BamHI site found at 739 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 756
Illegal XbaI site found at 1236
Illegal SpeI site found at 84 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 717
In the figures are the FACS test results of our parts., which are EBFP2 without and with intein respectively. The EBFP-A signals tell us that the split florescent proteins could fuse together and emit florescent light.
Without Dox, constructs containing CMV show no florescent signal, which means that one single N- or C- terminal cannot light up. However, once the other half is induced, they bind and emit light.