Difference between revisions of "Part:BBa K2139014"
 
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<partinfo>BBa_K2139014 short</partinfo>
 
<partinfo>BBa_K2139014 short</partinfo>
  
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Gluc1C is a monomeric enzyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
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This part encompass the coding region for an endo-beta-1,4-glucanase known as G12 and a double terminator. G12 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is ABK52392.1 (amino acid) and a PDB code of 1H0B_A (homolog).
  
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Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154
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Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 20:36, 30 October 2016


g12 with Terminator

This part encompass the coding region for an endo-beta-1,4-glucanase known as G12 and a double terminator. G12 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is ABK52392.1 (amino acid) and a PDB code of 1H0B_A (homolog).

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
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