Difference between revisions of "Part:BBa K2139010"
 
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<partinfo>BBa_K2139010 short</partinfo>
 
<partinfo>BBa_K2139010 short</partinfo>
  
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Gluc1C is a monomeric enzyme capable of catalyzing the conversion of cellobiose into monomeric glucose at the non reducing end of the sugar polymer. It can be used in conjunction with an endoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
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Ba_K2139010 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with a double terminator. Gluc1C is a monomeric enzyme capable of catalyzing decomposition of cellobiose down to two glucose monomers. It can be used in conjunction with an exo-beta-1,4-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.
  
 
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154
 
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Latest revision as of 21:04, 30 October 2016


Gluc1C with Terminator

Ba_K2139010 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with a double terminator. Gluc1C is a monomeric enzyme capable of catalyzing decomposition of cellobiose down to two glucose monomers. It can be used in conjunction with an exo-beta-1,4-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1138