Difference between revisions of "Part:BBa K2066054:Design"

 
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===Design Notes===
 
===Design Notes===
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to be completed
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This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. It should be used with the control part (no RiboJ sequence) K2066055.
  
  
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===Source===
 
===Source===
  
−
to be completed
+
The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added. The RiboJ sequence is from  Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"),
  
 
===References===
 
===References===

Latest revision as of 21:40, 16 October 2016


T7 Promoter with RiboJ Promoter Characterization Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. It should be used with the control part (no RiboJ sequence) K2066055.


Source

The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added. The RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"),

References