Difference between revisions of "Part:BBa K2066034"

Latest revision as of 16:31, 16 October 2016

mCherry + Spinach, J23107 + B0034

This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. Spinach and mCHerry allow characterization of gene expression at the RNA and Protein level, respectively. The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB and Spinach aptamer sequence are taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 1052
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 47
Illegal NheI site found at 70
Illegal SpeI site found at 1052
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 1052
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 1052
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2066034 short</partinfo>
-to be replaced
+This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. Spinach and mCHerry allow characterization of gene expression at the RNA and Protein level, respectively.  The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB and Spinach aptamer sequence are taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.
 <!-- Add more about the biology of this part here