Difference between revisions of "Part:BBa I715002:Design"

 
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<partinfo>BBa_I715002 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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This part is in plasmid pSB1A2. We plan to also build the part in the insulated plasmid pSB1A7 to eliminate the effects of read-through transcription.
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This part is in plasmid pSB3K3. We moved this insert from pSB1A2 (BBa_S03736) so that it would act as a good control for our Cre Reporter construct which will also be in pSB3K3.
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===Source===
 
===Source===

Latest revision as of 14:53, 24 July 2007

pLac-lox-RBS-Tet (in pSB3K3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 952
    Illegal NgoMIV site found at 1112
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in plasmid pSB3K3. We moved this insert from pSB1A2 (BBa_S03736) so that it would act as a good control for our Cre Reporter construct which will also be in pSB3K3.

Source

None

References