Difference between revisions of "Part:BBa K1943019:Design"

 
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===Design Notes===
 
===Design Notes===
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This TetOn expression system contains a SV40 promoter, a puro anti-antibiotics gene, 2A and TetOn 3G promoter. We design primers and do PCR of this TetOn expression system and ligate it to pSB1C3 backbone.
 
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===Source===
 
===Source===
  
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The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.
  
 
===References===
 
===References===
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#[[About_Assembly|Parts Registry Assembly Help]]
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#[[Help:Protocols/Transformation|Parts Registry Transformation Guideline]]

Latest revision as of 03:29, 22 October 2016


TetOn


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857


Design Notes

This TetOn expression system contains a SV40 promoter, a puro anti-antibiotics gene, 2A and TetOn 3G promoter. We design primers and do PCR of this TetOn expression system and ligate it to pSB1C3 backbone.

Source

The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline