Difference between revisions of "Part:BBa K1943013"

 
 
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<partinfo>BBa_K1943013 short</partinfo>
 
<partinfo>BBa_K1943013 short</partinfo>
  
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In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.
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The gene puro is corresponding to the antibiotic puromycin, which is an antibiotic that is a protein synthesis inhibitor by inhibiting translation.  Puromycin is selective for either prokaryotes or eukaryotes, but poorly active for E.coli.  We usually use 3μg/mL in cell culture.
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[[File:T--SUSTech Shenzhen--puro_1.svg | figure: the structure and Space-filling model of the puromycin molecule | 700px]]
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[[File:antibiotics from 2016Sustech biobricks.jpeg | 700px]]<br>
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Fig.2 Different concentration test of puromycin <br>
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(WT: CHO-K1 wild type, Piezo1: CHO-K1 transfected with puromycin resistant plasmid)
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:24, 22 October 2016


Puro

In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.

The gene puro is corresponding to the antibiotic puromycin, which is an antibiotic that is a protein synthesis inhibitor by inhibiting translation. Puromycin is selective for either prokaryotes or eukaryotes, but poorly active for E.coli. We usually use 3μg/mL in cell culture.

Error creating thumbnail: File missing

Antibiotics from 2016Sustech biobricks.jpeg
Fig.2 Different concentration test of puromycin
(WT: CHO-K1 wild type, Piezo1: CHO-K1 transfected with puromycin resistant plasmid)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481