Difference between revisions of "Part:BBa K1896012"

 
 
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This part was designed to express the Streptavidin molecule on the cell surface of ''E. coli''. Fusion proteins containing the [[Part:BBa_K811003|INP<sub>NC</sub>]] domain have been described to be directed to the outer cell membrane. [1] However, it appears that the tetrameric nature of Streptavidin makes the resulting fusion protein toxic to the cell, as no correct colonies could be obtained after multiple attempts.
  
 
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<partinfo>BBa_K1896012 parameters</partinfo>
 
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===References===
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<li>Wu, P. H., Giridhar, R., &amp; Wu, W. T. (2006). Surface display of transglucosidase on ''Escherichia coli'' by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. ''Biotechnology and bioengineering'', 95(6), 1138-1147.</li>
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</ol>

Latest revision as of 14:10, 19 October 2016


INP_NC-streptavidin generator

This part was designed to express the Streptavidin molecule on the cell surface of E. coli. Fusion proteins containing the INPNC domain have been described to be directed to the outer cell membrane. [1] However, it appears that the tetrameric nature of Streptavidin makes the resulting fusion protein toxic to the cell, as no correct colonies could be obtained after multiple attempts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1082
    Illegal AgeI site found at 1133
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Wu, P. H., Giridhar, R., & Wu, W. T. (2006). Surface display of transglucosidase on Escherichia coli by using the ice nucleation protein of Xanthomonas campestris and its application in glucosylation of hydroquinone. Biotechnology and bioengineering, 95(6), 1138-1147.