Difference between revisions of "Part:BBa K2020042:Experience"

Latest revision as of 21:26, 13 October 2016

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2020042

[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]

The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)

Y-RS
oNBY-RS
AzF
CN-F synthetase
Iodo-Y synthetase
5HT-P synthetase
Nitro-Y synthetase
Amino-Y synthetase

[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]

iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.

[http://2016.igem.org/Team:Aachen/Description iGEM-Team Aachen 2016 - LIPS Light inducible proteases]

iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and in E.coli BL21 DE3 gold. Furthermore they used the tRNA with the pFRY reporter for screening their newly designed DMNBS-RS.

User Reviews

UNIQa2e0ac51c4c29f6a-partinfo-00000000-QINU UNIQa2e0ac51c4c29f6a-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2020042===
-====[http://2014.igem.org/Team:Austin_Texas iGEM-Team Austin, Texas 2014]====
+====[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]====
-*[http://2014.igem.org/Team:Austin_Texas/kit Expanded Genetic Code Measurement Kit]
 The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)
 *Y-RS
@@ Line 17: / Line 16: @@
 *Amino-Y synthetase
-*[http://2014.igem.org/Team:Austin_Texas/photocage Project: Photocaged T7 RNA Polymerase]
+====[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]====
+iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.
-====[http://2016.igem.org/Team:Aachen iGEM-Team Aachen 2016]====
+====[http://2016.igem.org/Team:Aachen/Description iGEM-Team Aachen 2016 - LIPS Light inducible proteases]====
-*[http://2016.igem.org/Team:Aachen/Description LIPS - Light inducible proteases]
+iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and in E.coli BL21 DE3 gold. Furthermore they used the tRNA with the pFRY reporter for screening their newly designed DMNBS-RS.
-iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and DMNB-S in E.coli BL21 DE3 gold with their newly designed DMNBS-RS
 ===User Reviews===
@@ Line 36: / Line 35: @@
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K2020042 EndReviews</partinfo>
-Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.
-[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.