Difference between revisions of "Part:BBa K1941003"

 
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K1941003 short</partinfo>
 
<partinfo>BBa_K1941003 short</partinfo>
  
−
Scaffold RNA that targets the region c6 of CYC1 promoter and recruits the protein repressor module PCP-Mxi via the effector protein recruitments site PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and the PCP-binding RNA hairpin, PP7.
+
Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter
 +
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 23:09, 18 October 2016


scCyc1_PP7

Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter


Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]