Difference between revisions of "Part:BBa K2120323"

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<partinfo>BBa_K2120323 short</partinfo>
 
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J23119、J23116、J23109、J23106 are all constitutive promoter ,CI is an inhibitor which is built to repress the expression of PR promoter. The function is similar with the Part[BBa_K2120321]. The expression efficiency of CI depends on the concentration of plasmid within limits. So, we want to control the RBS active to influence the expression efficiency of inhibitor.
 
  
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J23116 is medium weak constitutive promoter，''cI'' is an inhibitor which is built to repress the activity of pR promoter. We linked two elements by medium ribosome binding site(RBS) B0032 to build this device. The expression efficiency of ''cI'' depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.
 
   
 
   
  

Latest revision as of 18:37, 22 October 2016


J23116+B0032+CI+B0015

J23116 is medium weak constitutive promoter，cI is an inhibitor which is built to repress the activity of pR promoter. We linked two elements by medium ribosome binding site(RBS) B0032 to build this device. The expression efficiency of cI depends on the concentration of plasmid within limits. So, we want to control the promoter strength to influence the expression efficiency of inhibitor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]