Difference between revisions of "Part:BBa K2110101"
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<partinfo>BBa_K2110101 short</partinfo> | <partinfo>BBa_K2110101 short</partinfo> | ||
| − | This is one of the site-directed mutant of the PETase gene, we change the | + | This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. |
Sequence and Features | Sequence and Features | ||
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===Results=== | ===Results=== | ||
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | ||
| − | https://static.igem.org/mediawiki/parts/thumb/ | + | https://static.igem.org/mediawiki/parts/thumb/e/e9/T--Tianjin--s207tnew.png/800px-T--Tianjin--s207tnew.png |
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 17:06, 19 October 2016
PETase site-directed mutant S207T
This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
Illegal BglII site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
