Difference between revisions of "Part:BBa K2110101"

Latest revision as of 17:06, 19 October 2016

PETase site-directed mutant S207T

This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. Sequence and Features

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 79
Illegal BglII site found at 289
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2110101 short</partinfo>
-This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
+This is one of the site-directed mutant of the PETase gene, we change the 207th amino acid from S to T. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
 Sequence and Features
@@ Line 12: / Line 12: @@
 ===Sequence and Features===
 <partinfo>BBa_K2110101 SequenceAndFeatures</partinfo>
-Activity Assay<br/>
+===Results===
 We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
-https://static.igem.org/mediawiki/parts/thumb/7/7a/T--Tianjin--partdataI208V.png/800px-T--Tianjin--partdataI208V.png
+https://static.igem.org/mediawiki/parts/thumb/e/e9/T--Tianjin--s207tnew.png/800px-T--Tianjin--s207tnew.png
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