Difference between revisions of "Part:BBa K2150112"

Latest revision as of 04:04, 20 October 2016

This part is used to build a kill-switch.

We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.

The growth curve of wild type E. coli harboring empty vector is shown below.

Wee also studied the interaction between toxin and antitoxin. See: BBa_K2150116

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 240
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 486
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 240
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 240
Illegal NgoMIV site found at 417
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
+<h2>toxin N-acetyltransferase GCN5 induced by aTc</h2>
-__NOTOC__
+===Usage and Biology===
-<partinfo>BBa_K2150112 short</partinfo>
-a
+This part is used to build a kill-switch.
-<!-- Add more about the biology of this part here
+===Characterization===
-===Usage and Biology===
+<h4>Growth Curve</h4>
+We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.
+[[file:T--UCAS--1204t-atc.png|500px]]
+The growth curve of wild type E. coli harboring empty vector is shown below.
+[[file:T--UCAS--RGP_Ptet.png|500px]]
+Wee also studied the interaction between toxin and antitoxin. See:
+[https://parts.igem.org/Part:BBa_K2150116 BBa_K2150116]
 <!-- -->