Difference between revisions of "Part:BBa K2150111"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | This part is used to build a kill-switch. | ||
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+ | ===Characterization=== | ||
+ | <h4>Growth Curve</h4> | ||
+ | We measured the growth curve of bacteria expressing toxin ParE2. | ||
+ | |||
+ | [[file:T--UCAS--6249t-IPTG.png|500px]] | ||
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+ | The growth curve of wild type E. coli harboring empty vector is shown below. | ||
+ | |||
+ | [[file:T--UCAS--RGP_Ptac.png|500px]] | ||
+ | |||
+ | We also studied the interaction between ParE2 and its corresponding antitoxin ParD2. See: | ||
+ | [https://parts.igem.org/Part:BBa_K2150117 BBa_K2150117] | ||
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Latest revision as of 03:43, 20 October 2016
Contents
toxin ParE2 induced by IPTG
Usage and Biology
This part is used to build a kill-switch.
Characterization
Growth Curve
We measured the growth curve of bacteria expressing toxin ParE2.
The growth curve of wild type E. coli harboring empty vector is shown below.
We also studied the interaction between ParE2 and its corresponding antitoxin ParD2. See: BBa_K2150117
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1848
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]