Difference between revisions of "Part:BBa K1995016"

 
 
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<partinfo>BBa_K1995015 short</partinfo>
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<partinfo>BBa_K1995016 short</partinfo>
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Based on BBa_K1355003 (merR), we added INP-N to the upstream of merR. Using INP-N, we anchored merR out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions.
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==Ice nucleation protein (INP)==
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To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merR out of the outer membrane of E. coli.
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Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.
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[[File:INP.png]]
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==Binding heavy metals==
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We detect the reducing efficiency of IR and other parts and get the following chart. (Fig. 2)
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[[File:0162.png]]
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BR (merR coding sequence)
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1995016 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1995015 parameters</partinfo>
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<partinfo>BBa_K1995016 parameters</partinfo>
 
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Latest revision as of 18:50, 15 October 2016


IR (INP-N-merR)

Based on BBa_K1355003 (merR), we added INP-N to the upstream of merR. Using INP-N, we anchored merR out of the outer membrane of E.coli in order to increase the reducing efficiency of mercuric ions.

Ice nucleation protein (INP)

To increase the reducing efficiency and detect the concentration of heavy metals, we used INP to anchor merR out of the outer membrane of E. coli.

Ice nucleation protein (INP) is an excretion surface protein. It is widely used for establishing germ surface display system. It contains an internal repeated domain (IRD) and an N-terminal anchoring domain (fig.1). Studies have shown that decrease the time of repetition of IRD can also get a better-off rate of displaying. So we decreased the time of repetition of IRD to reduce pressure of synthesis of E. coli.

INP.png

Binding heavy metals

We detect the reducing efficiency of IR and other parts and get the following chart. (Fig. 2)

0162.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238