Difference between revisions of "Part:BBa K1976025"
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Orthogonal pair for amber supression with OMT | Orthogonal pair for amber supression with OMT | ||
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| + | <p>An orthogonal pair for <i>O</i>‑methyl‑<span style="font-variant:small-caps">l</span>‑tyrosine incorporation at the position of the <i>amber</i> stop codon. The pair ist based on the pairs from the <a href="http://2014.igem.org/Team:Austin_Texas/kit">"Expanded Genetic Code Measurement Kit"</a> by the iGEM team Austin Texas 2014. The <i>open reading frame</i> (ORF) of the aminoacyl tRNA synthetase was exchanged with the ORF of the OMT tRNA synthtase <a href="https://parts.igem.org/Part:BBa_K1976020">BBa_K1976020</a>.</p> | ||
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Latest revision as of 14:41, 22 October 2016
Orthogonal pair for amber supression with OMT
Orthogonal pair for amber supression with OMT
An orthogonal pair for O‑methyl‑l‑tyrosine incorporation at the position of the amber stop codon. The pair ist based on the pairs from the "Expanded Genetic Code Measurement Kit" by the iGEM team Austin Texas 2014. The open reading frame (ORF) of the aminoacyl tRNA synthetase was exchanged with the ORF of the OMT tRNA synthtase BBa_K1976020.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1153
Illegal BamHI site found at 1159 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1185
Illegal NgoMIV site found at 1645 - 1000COMPATIBLE WITH RFC[1000]