Difference between revisions of "Part:BBa K1937003"
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<partinfo>BBa_K1937003 short</partinfo> | <partinfo>BBa_K1937003 short</partinfo> | ||
− | The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs | + | The BBa_K1937003 part was designed to demonstrate the induction of pNagA by N-AcetylGlucosamine-6-phosphate (NAG). It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). <br> |
− | pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis | + | pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in <i>Bacillus subtilis.</i> |
+ | This promoter has been reported to induce expression in presence of NAG. NAG molecule is the major component of chitin, a characteristic molecule of fungi. | ||
+ | <br> | ||
+ | [[File:BBa_K1937003_-construction.png]] | ||
<!-- Add more about the biology of this part here ===Usage and Biology===<!-- --><span class='h3bb'>Sequence and Features</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- --> | <!-- Add more about the biology of this part here ===Usage and Biology===<!-- --><span class='h3bb'>Sequence and Features</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- --> |
Latest revision as of 19:11, 17 October 2016
pNagA-RFP
The BBa_K1937003 part was designed to demonstrate the induction of pNagA by N-AcetylGlucosamine-6-phosphate (NAG). It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis.
This promoter has been reported to induce expression in presence of NAG. NAG molecule is the major component of chitin, a characteristic molecule of fungi.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 201
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 761
Illegal AgeI site found at 873 - 1000COMPATIBLE WITH RFC[1000]