Difference between revisions of "Part:BBa K1935005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | A mutagenesis was done to abolish an illegal restriction site ( | + | A mutagenesis was done to abolish an illegal restriction site (Pst1 : 425 to 430 pb, CTGCAG => CAGCAG) |
Latest revision as of 12:41, 13 October 2016
White collar-2 (WC-2) - photoreceptor in Neurospora tetrasperma
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 25
Illegal BamHI site found at 61
Illegal BamHI site found at 835 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1251
Illegal NgoMIV site found at 1310
Illegal AgeI site found at 857 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 402
Illegal BsaI.rc site found at 770
Illegal SapI.rc site found at 652
Design Notes
A mutagenesis was done to abolish an illegal restriction site (Pst1 : 425 to 430 pb, CTGCAG => CAGCAG)