Difference between revisions of "Part:BBa K1937003"

 
 
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<partinfo>BBa_K1937003 short</partinfo>
  
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The BBa_K1937003 part was designed to demonstrate the induction of pNagA by N-AcetylGlucosamine-6-phosphate (NAG). It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France). <br>
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<partinfo>BBa_K1937003 short</partinfo>
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pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in <i>Bacillus subtilis.</i>
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This promoter has been reported to induce expression in presence of NAG. NAG molecule is the major component of chitin, a characteristic molecule of fungi.  
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The BBa_K1937003 part was designed to demonstrate the induction of pNagA by NAG. It belongs tothe antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
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<br>
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pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis  
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[[File:BBa_K1937003_-construction.png]]
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In bacillus subtilis, this promoter has been reported to induce expression in presence of N-acetylglucosamine 6 phosphate.
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<!-- Add more about the biology of this part here ===Usage and Biology===<!-- --><span class='h3bb'>Sequence and Features</span> <partinfo>BBa_K1937003 SequenceAndFeatures</partinfo> <!-- Uncomment this to enable Functional Parameter display ===Functional Parameters=== <partinfo>BBa_K1937003 parameters</partinfo> <!-- -->
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This NAG molecule is the major component of chitin, a characteristic molecule of fungi.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1937003 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
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===Functional Parameters===
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<partinfo>BBa_K1937003 parameters</partinfo>
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Latest revision as of 19:11, 17 October 2016

pNagA-RFP

The BBa_K1937003 part was designed to demonstrate the induction of pNagA by N-AcetylGlucosamine-6-phosphate (NAG). It belongs to the antifungal module in the Paleolitis project of iGEM Toulouse 2016 (http://2016.igem.org/Team:Toulouse_France).
pNagA is the promoter of the NagA gene, which expresses a N-acetylglucosamine-6-phosphate deacetylasein in Bacillus subtilis. This promoter has been reported to induce expression in presence of NAG. NAG molecule is the major component of chitin, a characteristic molecule of fungi.
BBa K1937003 -construction.png Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 201
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]