Difference between revisions of "Part:BBa K1997015:Design"

 
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===Design Notes===
 
===Design Notes===
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This part is designed to be a positive control which contains the complete ZFN protein. Compared with the experimental groups, it will be easily to find the disparity between them.
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We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced.
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+
−
 
+
  
 
===Source===
 
===Source===
  
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We obtained it through the contribution of our basic subparts.
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This part is synthesized by company.
  
 
===References===
 
===References===

Latest revision as of 00:00, 14 October 2016


P+R->sGFP-N->Zif268->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 832
    Illegal AgeI site found at 916
    Illegal AgeI site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1003
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1217


Design Notes

We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced.

Source

This part is synthesized by company.

References