Difference between revisions of "Part:BBa K2150028:Design"
| Line 19: | Line 19: | ||
pT7:PCR amplified from BBa_K525998. | pT7:PCR amplified from BBa_K525998. | ||
| − | As for fusion protein tetX-GFP, tetX is the same as | + | As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR). |
===References=== | ===References=== | ||
Latest revision as of 23:53, 19 October 2016
pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3406
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4703
Design Notes
Two different terminators are used to avoid repeated sequence.
Linker for fusion protein is carefully designed to avoid repeated sequence which may cause problems in assembly.
Source
T7RNAP:PCR amplified from Genome of BL21.
pT7:PCR amplified from BBa_K525998.
As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).