Difference between revisions of "Part:BBa K2150028"

 
 
(3 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2150028 short</partinfo>
 
<partinfo>BBa_K2150028 short</partinfo>
  
−
In this part, the first promoter pTet is constitutively on, producing T7 RNA polymerase(T7RNAP). T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity. The fusion protein(referred to as tetX-GFP) of tetX(a tetracycline-degrading enzyme) and GFP combined with a 3*GGGGS linker is used to report the expression level of tetX.
+
In this part, T7 RNA polymerase(T7RNAP) is expressed from a tetracycline-inducible promoter pTet. T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity.  
 +
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
We design this part to further enhance the degrading capacity of our bacteria. The amount of tetX is reflected by the fluorescent intensity, and the degradation of tetracycline is indicated by the survival and growth of the cell.
 +
 +
===Characterization===
 +
[[File:Captain Scavenger.png|500 px|thumb|left]]
 +
 +
 +
As shown in Fig.1, compariing to [https://parts.igem.org/Part:BBa_K2150015 BBa_K2150015], this part proforms better in the presence of tetR, at all Tc concentration applied. Bacteria carrying this part grow better, and produce a larger amount of degrading-enzyme (represented by fluorescence intensity) in all than the Bacteria carrying BBa_K2150015.
 +
 +
   
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 01:16, 20 October 2016


pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT

In this part, T7 RNA polymerase(T7RNAP) is expressed from a tetracycline-inducible promoter pTet. T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity.


Usage and Biology

We design this part to further enhance the degrading capacity of our bacteria. The amount of tetX is reflected by the fluorescent intensity, and the degradation of tetracycline is indicated by the survival and growth of the cell.

Characterization

Captain Scavenger.png


As shown in Fig.1, compariing to BBa_K2150015, this part proforms better in the presence of tetR, at all Tc concentration applied. Bacteria carrying this part grow better, and produce a larger amount of degrading-enzyme (represented by fluorescence intensity) in all than the Bacteria carrying BBa_K2150015.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4703