Difference between revisions of "Part:BBa K2150024"

Latest revision as of 03:45, 13 October 2016

pCon+RBS+tetR+DT+pTet+RBS+GFP+DT

The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of GFP and emission of green fluorescence. This part can be used to verify the tet-on/tet-off system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1615

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2150024 short</partinfo>
-The first promoter is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of GFP and emission of green fluorescence. This part can be used to verify the tet-on/tet-off system.
+The first promoter(BBa_J23119) is constitutively on and can express tetR, a protein that can repress the downstream promoter pTet in the absence of tetracycline(Tc). As a result, no GFP is expressed and no fluorescence can be detected. However, in the presence of Tc, tetR is repressed and repression over pTet is removed. This leads to the expression of GFP and emission of green fluorescence. This part can be used to verify the tet-on/tet-off system.
 <!-- Add more about the biology of this part here