Difference between revisions of "Part:BBa K2150019:Design"
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pT7 PCR amplified from BBa_K525998,pTet from BBa_R0040,RBS from BBa_B0034,DT from BBa_B0015 | pT7 PCR amplified from BBa_K525998,pTet from BBa_R0040,RBS from BBa_B0034,DT from BBa_B0015 | ||
| − | As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR). | + | |
| + | As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR). | ||
===References=== | ===References=== | ||
Latest revision as of 04:11, 13 October 2016
pT7+pTet+RBS+tetX-GFP(fusion protein)+DT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 670
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1967
Design Notes
Fusion protein is designed to report the accurate expression level of tetX. With pT7 promoter, expression of tetX-GFP can be amplified.
Source
pT7 PCR amplified from BBa_K525998,pTet from BBa_R0040,RBS from BBa_B0034,DT from BBa_B0015
As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).