Difference between revisions of "Part:BBa K1900000:Design"

 
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===Design Notes===
 
===Design Notes===
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BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Klebsiella pneumoniae TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be BioBrick compatible.
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===Source===
 
===Source===
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BBa_K1406000 comes from the BioBrick registry The tolC signaling sequence and Klebsiella pneumoniae tolC sequence were synthesized from DNA sequences found in genomic databases.
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This part
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===References===
 
===References===

Latest revision as of 16:52, 20 October 2016


pBAD+strong RBS+E. coli tolC signal sequence+K. pneumoniae tolC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1603
    Illegal NheI site found at 1624
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1104


Design Notes

BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Klebsiella pneumoniae TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be BioBrick compatible.

Source

BBa_K1406000 comes from the BioBrick registry The tolC signaling sequence and Klebsiella pneumoniae tolC sequence were synthesized from DNA sequences found in genomic databases.

References