Difference between revisions of "Part:BBa K1896003"

Latest revision as of 20:34, 18 October 2016

INP_NC (opt)

This is an E. coli codon optimised version of BBa_K811003, which encodes a truncated variant of an ice nucleating protein (INP) found in Xanthomonas campestris. The truncated protein INPNC lacks the large central repeat region, but retains membrane display abilities. The stopcodon and one BsaI site were removed from the recoded sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 405
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:50, 12 October 2016 (view source) Registry (Talk \| contribs)		Latest revision as of 20:34, 18 October 2016 (view source) Bvh (Talk \| contribs)
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	<partinfo>BBa_K1896003 short</partinfo>		<partinfo>BBa_K1896003 short</partinfo>

−	This is an ~~improved~~ version of [[Part:BBa_K811003\|BBa_K811003]], which encodes a truncated variant of an ice nucleating protein (INP) found in ''Xanthomonas campestris''. The truncated protein INPNC lacks the large central repeat region, but retains membrane display abilities. The stopcodon and one BsaI site ~~was~~ removed.	+	This is an <i> E. coli </i> codon optimised version of [[Part:BBa_K811003\|BBa_K811003]], which encodes a truncated variant of an ice nucleating protein (INP) found in ''Xanthomonas campestris''. The truncated protein INPNC lacks the large central repeat region, but retains membrane display abilities. The stopcodon and one BsaI site were removed from the recoded sequence.