Difference between revisions of "Part:BBa K2074014"
 
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<partinfo>BBa_K2074014 short</partinfo>
 
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2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure7. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas
 
LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP
 
LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP
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[[File:2016FAFUCHINADevice3.png|800px|thumb|center|'''Fig.1''' The Mechanism of 2A Peptide System]]
  
 
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Latest revision as of 08:10, 18 October 2016


Extended FMDV 2A for Chlamydomonas reinhardtii

2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure7. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP

Fig.1 The Mechanism of 2A Peptide System

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]