Difference between revisions of "Part:BBa K2110109"
 
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<partinfo>BBa_K2110109 short</partinfo>
 
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This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from A to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
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This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from S to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K2110109 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2110109 SequenceAndFeatures</partinfo>
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Activity Assay<br/>
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===Results===
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
 
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
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https://static.igem.org/mediawiki/parts/6/61/T--Tianjin--partA238F.png
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https://static.igem.org/mediawiki/parts/thumb/a/a1/T--Tianjin--partdataS238F.png/800px-T--Tianjin--partdataS238F.png
  
 
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Latest revision as of 17:37, 13 October 2016


PETase site-directed mutant S238F

This is one of the site-directed mutant of the PETase gene, we change the 238th amino acid from S to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--partdataS238F.png