Difference between revisions of "Part:BBa K2150016"

Latest revision as of 00:31, 20 October 2016

pT7 GFP

T7 promoter and RBS and GFP, with double terminator.

Usage and Biology

We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed a leakage of T7 promoter on high-copy plasmid pSB1C3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 690

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K2150015 short</partinfo>
+<partinfo>BBa_K2150016 short</partinfo>
-T7 promoter and RBS and GFP, with double terminators. Though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase.
+T7 promoter and RBS and GFP, with double terminator.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed a leakage of T7 promoter on high-copy plasmid pSB1C3.
+[[File:pT7+GFP.png|350px]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2150015 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2150016 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K2150015 parameters</partinfo>
+<partinfo>BBa_K2150016 parameters</partinfo>
 <!-- -->