Difference between revisions of "Part:BBa K2150017:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Expression level of tetX can be measured by the fluorescence intensity of GFP and the expression of tetracycline-degrading enzyme tetX can be amplified by T7 RNA polymerase.
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Expression level of tetX can be measured by the fluorescence intensity of GFP and the expression of tetracycline-degrading enzyme tetX can be amplified by T7 RNA polymerase(not included here).
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+
−
 
+
  
 
===Source===
 
===Source===
  
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pT7 promoter with RBS comes from BBa_K525998,DT from BBa_B0015.  
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pT7 promoter comes from BBa_K525998, RBS from BBa_B0034, DT from BBa_B0015.  
−
As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).  
+
As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine these three parts by overlap extension polymerase chain reaction (OE-PCR).  
  
 
===References===
 
===References===

Latest revision as of 00:21, 20 October 2016


pT7 TetX-GFP(fusion protein)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1905


Design Notes

Expression level of tetX can be measured by the fluorescence intensity of GFP and the expression of tetracycline-degrading enzyme tetX can be amplified by T7 RNA polymerase(not included here).

Source

pT7 promoter comes from BBa_K525998, RBS from BBa_B0034, DT from BBa_B0015. As for fusion protein tetX-GFP, tetX is the same as BBa_K2150101, the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine these three parts by overlap extension polymerase chain reaction (OE-PCR).

References