Difference between revisions of "Part:BBa K1991000"

Latest revision as of 14:42, 19 October 2016

AOX1 (Alcohol oxidase)

Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide. The substrates of AOX are primary alcohols including methanol and some short-chain alcohols such as ethanol. AOX is present in the methylotrophic yeast like Pichia pastoris, which has two genes (i.e., AOX1 and AOX2) under a strong methanol inducible promoter. The yeast uses AOX genes to metabolize methanol for providing a carbon and energy source. The AOX enzymes were used in biosensors (Biosens Bioelectron. 2005).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1687

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 <partinfo>BBa_K1991000 short</partinfo>
-There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol.
+Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide. The substrates of AOX are primary alcohols including methanol and some short-chain alcohols such as ethanol. AOX is present in the methylotrophic yeast like Pichia pastoris, which has two genes (i.e., AOX1 and AOX2) under a strong methanol inducible promoter. The yeast uses AOX genes to metabolize methanol for providing a carbon and energy source. The AOX enzymes were used in biosensors ([https://www.ncbi.nlm.nih.gov/pubmed/16023950 Biosens Bioelectron. 2005]).
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