Difference between revisions of "Part:BBa K1980006:Design"

 
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<partinfo>BBa_K1980006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1980006 SequenceAndFeatures</partinfo>
  
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Designed so that any protein can be ligated to this part with standard assembly to make them respond to copper (for instance our copper chelator proteins)
  
 
===Design Notes===
 
===Design Notes===
−
Codon optimised. Designed for biobrick assembly with reporter of choice
+
Codon optimised. Designed for biobrick assembly with protein/reporter of choice.
  
  
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===Source===
 
===Source===
  
−
E. coli
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The promoter originates from <i>E. coli</i>. Ordered as codon optimised DNA from IDT.
  
 
===References===
 
===References===
 +
Yamamoto K, Ishihama A. (2005) “Transcriptional response of Escherichia coli to external copper.” Mol Microbiol. 2005 Apr;56(1):215-27.
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 +
Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472

Latest revision as of 23:00, 23 October 2016


pCopA with divergent expressed CueR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 438
    Illegal NheI site found at 461
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed so that any protein can be ligated to this part with standard assembly to make them respond to copper (for instance our copper chelator proteins)

Design Notes

Codon optimised. Designed for biobrick assembly with protein/reporter of choice.


Source

The promoter originates from E. coli. Ordered as codon optimised DNA from IDT.

References

Yamamoto K, Ishihama A. (2005) “Transcriptional response of Escherichia coli to external copper.” Mol Microbiol. 2005 Apr;56(1):215-27.

Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472