Difference between revisions of "Part:BBa K1980003:Design"
(→References) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. | + | This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. The sfGFP has been seperated from the MymT by a short linker (GlySerGlySerGlySer) to allow both domains to fold. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | Mycobacterium tuberculosis. | + | MymT is originally from <i>Mycobacterium tuberculosis</i>. We ordered it as codon optimised DNA from IDT. |
===References=== | ===References=== | ||
| + | Ben Gold, Haiteng Deng, Ruslana Bryk, Diana Vargas, David Eliezer, Julia Roberts, Xiuju Jiang, & Carl Nathan (2009) “Identification of a Copper-Binding Metallothionein in Pathogenic Mycobacteria” Nat Chem Biol. 2008 October ; 4(10): 609–616. doi:10.1038/nchembio.109. | ||
Latest revision as of 20:46, 23 October 2016
MymT sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 595
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli. The sfGFP has been seperated from the MymT by a short linker (GlySerGlySerGlySer) to allow both domains to fold.
Source
MymT is originally from Mycobacterium tuberculosis. We ordered it as codon optimised DNA from IDT.
References
Ben Gold, Haiteng Deng, Ruslana Bryk, Diana Vargas, David Eliezer, Julia Roberts, Xiuju Jiang, & Carl Nathan (2009) “Identification of a Copper-Binding Metallothionein in Pathogenic Mycobacteria” Nat Chem Biol. 2008 October ; 4(10): 609–616. doi:10.1038/nchembio.109.