Difference between revisions of "Part:BBa K2170051:Design"
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<partinfo>BBa_K2170051 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2170051 SequenceAndFeatures</partinfo> | ||
+ | <br>'''Keywords:''' | ||
+ | <!--These keywords are necessary to find your part using a fulltext sarch.--> | ||
+ | <!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5--> | ||
+ | |||
+ | <br>'''Abbreviations:''' | ||
+ | <!--*used_abbreviation_1 = full_name_of_used_abbreviations_1--> | ||
+ | <!--*used_abbreviation_2 = full_name_of_used_abbreviations_2--> | ||
===Design Notes=== | ===Design Notes=== | ||
− | |||
+ | '''Related BioBrick:''' | ||
+ | <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] --> | ||
+ | <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] | ||
+ | '''Cloning details:'''<br> | ||
+ | <!--*Designed in RFC10/RFC23/RFC25--> | ||
+ | <!--*Mutation C889G to delete XbaI restriction site--> | ||
+ | <!--*Truncation upstream/downstream compared to template, ?explanation?--> | ||
+ | |||
+ | '''Quality control measures:'''<br> | ||
+ | <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed--> | ||
+ | <!--*Sequencing using primer ?primer_name?/Not yet sequenced--> | ||
+ | <!--*Part was partly sequenced/Part was totally sequenced--> | ||
+ | |||
+ | '''Backbone:'''<br> | ||
+ | <!--*Backbone name: pSB1C3'/?backbone_name?--> | ||
+ | <!--*Resistance: Amp/Cp/Kan/--> | ||
+ | <!--*Copynumber: low/medium/high--> | ||
+ | |||
+ | '''Protein coding:'''<br> | ||
+ | <!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]--> | ||
+ | <!--*The protein has the amino acid replacements ???99??? to ???99???.--> | ||
+ | <!--*The protein encoded is posttranslationally modified by ???.--> | ||
+ | <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other--> | ||
+ | |||
+ | '''Enzymatic activity:''' | ||
+ | <!--none/EC-number ?.?.?.?--> | ||
+ | |||
+ | '''Cytotoxicity:'''<br> | ||
+ | <!--none/not known/cytotoxic for ''organism name''--> | ||
+ | |||
+ | '''Safety notes:'''<br> | ||
+ | <!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk--> | ||
+ | <!--Known and anticipated security issues: none/other.--> | ||
+ | |||
+ | '''Intellectual property:''' | ||
+ | <!--Information on patent situation.--> | ||
+ | <!--Intellectual property claims made by the authors.--> | ||
+ | |||
+ | '''Corresponding part author/authors:''' | ||
+ | <!--https://igem.org/User_Information.cgi?user_id=????/email--> | ||
===Source=== | ===Source=== | ||
− | + | '''Source:'''<br> | |
+ | <!--*Commercial system: plasmid name, system name, company name--> | ||
+ | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
+ | <!--*Preexisting BioBrick ?Bba_number?--> | ||
+ | <!--*cDNA Clone: ?clone_name?, ?company_name?--> | ||
+ | <!--*Synthesized by ?company_name?.--> | ||
+ | |||
+ | <!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
+ | <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>--> | ||
+ | |||
+ | '''Organism:'''<br> | ||
+ | Genesequence derived from ''Escherichia coli'' | ||
+ | <!--*Codonoptimized for ''?organism_name?''--> | ||
+ | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
+ | <!--*Statement about functionality in other chassis.--> | ||
+ | |||
+ | <!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []--> | ||
===References=== | ===References=== | ||
+ | <!-- Here you find templates to insert references to literature and different databases--> | ||
+ | |||
+ | '''Literature references:'''<br> | ||
+ | 1. [http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]<br> | ||
+ | 2. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]<br> | ||
+ | 3. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]<br> | ||
+ | 4. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754705/ Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.]<br> | ||
+ | 5. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]<br> | ||
+ | |||
+ | |||
+ | '''Database references:'''<br> | ||
+ | <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]--> | ||
+ | <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | ||
+ | <!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]--> | ||
+ | <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]--> | ||
+ | <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]--> | ||
+ | <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]--> |
Latest revision as of 16:02, 19 October 2016
Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1167
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2625
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 889
Illegal BsaI.rc site found at 1149
Keywords:
Abbreviations:
Design Notes
Related BioBrick:
Quality control measures:
Backbone:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Organism:
Genesequence derived from Escherichia coli
References
Literature references:
1. [http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]
2. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
3. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
4. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
5. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]
Database references: