Difference between revisions of "Part:BBa K1968000:Design"
 
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===Design Notes===
 
===Design Notes===
−
The sequence of this part did not need to be re-designed. This part contains two MoClo fusion sites, A at the 5' end and B at the 3' end
+
The sequence of this part did not need to be re-designed. This part contains two Phytobrick fusion sites, A at the 5' end and B at the 3' end
  
 
A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
 
A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Latest revision as of 16:52, 17 October 2016


PA1lac0-1 Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part did not need to be re-designed. This part contains two Phytobrick fusion sites, A at the 5' end and B at the 3' end

A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Source

This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing”

References