Difference between revisions of "Part:BBa K2088016"

Latest revision as of 19:25, 19 October 2016

nativeRBS-pbaA1A2B with a double Terminator

pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA). Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1]

In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2648
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 116
Illegal NgoMIV site found at 776
Illegal NgoMIV site found at 1383
Illegal NgoMIV site found at 1407
Illegal AgeI site found at 1291
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2088016 short</partinfo>
-This part includes optimized mhbI (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator). We choose Escherichia coli K12 as the organism to optimize the sequences.
+pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA).
+Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1]
+In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015.
 <!-- Add more about the biology of this part here